transfection

美 [træns'fekʃən]英 [træns'fekʃən]
  • n.转染;转变感染;转移感染;传染
  • 网络转染作用;基因转染;细胞转染

transfectiontransfection

transfection

转染

转染(transfection):1.感受态细菌细胞受无外壳的噬菌体DNA的感染,发生转化作用,然后产生忧感染性的噬菌体.只是DNA而无蛋 …

转染作用

(1)转染作用(transfection)把λ噬菌体改造成为基因工程中用的载体,目的是把外源DNA片段插入载体中,并使它导入受体细胞.最简 …

转移感染

然而转移感染 (transfection) 和 HES 细胞的选择等技术仍需要更进一步的改良,方能增加机会得到经基因改造以符合免疫配对 …

基因转染

用野生型P53基因转染(transfection)治疗由P53变异而产生的肿瘤,或表达异常P53基因的肿瘤。  (2) P21基因。

细胞转染

分子生物学研究中心 ... 二倍体( diploid) 细胞转染( transfection) 细胞转化( transformation) ...

转染法

... 通过病毒把无毒性DNA转移至一个细胞中 3)转染法(transfection) – 把DNA转移至动物细胞中的过程 五、基因组文库克隆子 …

转染细胞

七、转染细胞(Transfection)---------------------------------------------21八、西方墨点法(Western Blot Analysis)---------------...

转染技术

七、细胞转染技术 (Transfection) 19 八、分析mRNA表现 (Semi-quantitative -PCR) 20 (一)、萃取RNA 20 (二)、反转录mRNA 2…

1
Immediately after gene transfection, blood flow of the internal iliac artery had no significant difference between three groups. 术后立即测定的髂内动脉血流量各组间无明显差异。
2
Transfection is the process of introducing DNA or RNA into a cell without using viruses to deliver them. 转染是指不用病毒而直接将DNA或者RNA导入体细胞。
3
Methods: Experiment was divided into three groups: negative control group, blank plasmid group, interference fragment transfection group. 方法:实验分三组:阴性对照组,空白质粒转然组,干扰片段转染组。
4
abstract: Objective: To discuss cells with different initial inoculation density on transfection efficiency. 目的:探讨不同初始接种密度对细胞转染效率的影响。
5
The invention relates to compositions of transfection comprising an oligonucleotide active for RNA interference. 本发明涉及包括具有RNA干扰活性的寡核苷酸的转染组合物。
6
CONCLUSION: In vitro perfusion during graft cold preservation is feasible for adenovirus mediated gene transfection. 结论:小鼠心脏移植保存期间体外灌注可应用于腺病毒基因转染等移植物处理过程。
7
ObjectiveTo evaluate the efficiency for eukaryotic cell transfection by using GFP as a report gene in common transfection ways. 目的比较常见的真核细胞转染技术,评价采用绿色荧光蛋白为报告基因来观察细胞转染效率高低的方法。
8
Gene transfection was a technology of transfecting the exogenous genes into the eukaryotic cells. 基因转染技术是将外源基因转染真核细胞的一种技术。
9
In cases where more than one transfection reagent can be used, we highlight the reagents we recommend for most robust performance. 在能够使用一种以上转染试剂的情况中,我们对能显示强健性能的试剂进行标记。
10
Cell morphological changes were investigated by reverse microscopy before and after transfection. 倒置显微镜下观察转染前后细胞形态学的改变;
11
Moreover, an involvement of the GGT promoter II was detected after RT-PCR and transient transfection studies. 此外,参与谷氨酰转移酶启动子二是发现后RT-PCR和瞬时转染研究。
12
Results No significant difference was found in the transformation rate of liver cells for lidocaine before and after SV40 gene transfection. 结果转染前、后肝细胞中利多卡因的浓度两者的利多卡因转化率无明显差异;
13
This evidence is based largely on transfection of cells with collagen-reporter gene constructs and on studies of transgenic mice. 这方面的证据主要根据转染的细胞胶原基因结构的研究和转基因小鼠。
14
The original chitosan carrier is modified to improve the transfection efficiency and the targeted property to respiratory cells. 对原始壳聚糖载体进行改性,提高其转染效率和对于呼吸道细胞的靶向性。
15
Conclusion: The establishment of MDR cell lines by gene transfection is an ideal method. 结论:基因转染法是建立人膀胱癌多药耐药细胞模型的理想方法。
16
Conclusion: PTEN gene transfection combined with L-OHP has obvious inhibitory effect on cholangiocarcinoma cell line in vitro. 结论:PTEN基因转染生物治疗联合奥沙利铂化疗对人胆管癌细胞生长具有显著的抑制作用。
17
The transfection efficiency decrease with the increase of the alkyl chains. 随着碳链长度的增加,表面活性剂的转染效率随之降低;
18
Grow L929 cell to ~80% confluency at the day of transfection. L929 cell must be healthy and less than 9 passages for maximum efficiency. 转染时确保L929细胞达到80%的融合度,细胞必须健康并且传代不能超过9代。
19
This article reviews about progress in gene transfection technique to myocardium i. . . 本文仅就目前体内心肌基因转染技术的进展作一综述。
20
Transfection of NF- B decoy suppressed proliferation of human coronary artery smooth muscle cells in vitro. NF-B引诱物转染抑制活体外的人冠状动脉平滑肌细胞增殖。
21
Positive Ion Lipoplexes and Its Application in Gene Transfection and Gene Therapy. 阳离子脂质体及其在基因转移和基因治疗中的应用。
22
Also, no significant difference was observed in ultra-structure of liver cells under electron microscope before and after transfection. 电镜下比较转染前、后的正常肝细胞的超微结构亦无统计学差异。
23
Second, in isolated rat adipose cells, glucose transporter 4 trafficking can be visualized by transfection of HA-GLUT4-GFP. 然后,在大鼠的脂肪细胞中,通过转染HA-GLUT4-GFP来观察葡萄糖转运蛋白4的转运过程。
24
Transfection with full-length VP15 resulted in GFP fluorescence being distributed exclusively in the nucleus. 在转染全长VP15基因的细胞里,荧光信号只分布在细胞核内。
25
The inhibitory effect of gene transfection on solid tumor was observed in mice. 小鼠实体瘤块模型研究基因转染抑制肝癌生长作用。
26
Transfection rate was measured by flow cytometry. 流式细胞仪检测转染效率;
27
Conclusions C2 gene transfection can lead SGC7901 cells to apoptosis, but the mechanism has to be further study. 结论C2基因转染可引起胃癌细胞发生凋亡,机制有待进一步研究。
28
Luciferase activities were measured 72 hr post-transfection. 转染72小时后测量荧光素酶的活性。
29
Gene transfection is the key technique of gene therapy. 基因治疗的关键技术是基因转移。
30
Nevertheless, with the increasing of transfection rate, worried about safety problems such as gene integration were worse. 然而,转染效率的增加,使得人们对基因整合等安全性问题的担心更甚。